Protocols
DNA Methylation Protocols
- Bisulfite Treatment of DNA (Issa Lab, Department of Leukemia at the MD Anderson Cancer Center)
- Methylation-Sensitive PCR (MSP) (Issa Lab, Department of Leukemia at the MD Anderson Cancer Center)
- Bisulfite-PCR (for restriction and/or sequencing) (Issa Lab, Department of Leukemia at the MD Anderson Cancer Center)
- Southern Blot Analysis (Issa Lab, Department of Leukemia at the MD Anderson Cancer Center)
- Methylated CpG Island Amplification (MCA) (Issa Lab, Department of Leukemia at the MD Anderson Cancer Center)
- DNA-Methyltransferase Assay (Issa Lab, Department of Leukemia at the MD Anderson Cancer Center)
- Bisulfite
Modification (Technique Tips Online, BioMedNet)
Modify DNA with sodium bisulfite for methylation analysis. - Methylation
Detection after Restriction (Susan E Andrew, Medical
Genetics, University of Alberta)
Digest DNA with methylation sensitive enzymes and subsequently do PCR to detect DNA methylation. - RLGS
protocol (Makoto Kawase)
Detailed Restriction Landmark Genomic Scanning (RLGS) protocol for global methylation study. Although this protocol is for plant genome, it can also be applied to other genome. This technique has been used for genome-wide methylation analysis and has the name of Restriction Landmark Genomic Scanning for Methylation (RLGS-M).
References describing the original bisulfite based methylation mapping methods
- Clark SJ, Harrison J, Paul CL, Frommer M. High sensitivity mapping of methylated cytosines.
- Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.