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Welcome to MethPrimer!

MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primers. Currently, it can design primers for two types of bisulfite PCR: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR. MethPrimer can also predict CpG islands in DNA sequences

Input Sequence: A DNA sequences in any format. No editing is required before input.>

Output: MethPrimer returns results in both text and graphic view including results of primer picking and CpG island prediction.

Go to MethPrimer (plus CpG Island Prediction)

How to cite MethPrimer: Li LC and Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002 Nov;18(11):1427-31. PMID: 12424112.

MethPrimer has been cited over 1200 times according to Google Scholar. Click here to see who has used the MethPrimer program.

Submit your paper to Journal of Biological Methods (ISSN 2326-9901) - an open access journal