Paste an ORIGINAL source sequence. Try this sample sequence You do not need to modify your sequence (e.g. convert 'C's to 'T') before pasting. Pick primers for bisulfite sequencing PCR or restriction PCR. Pick MSP primers. Use CpG island prediction for primer selection? Window 100 200 300 400 Shift 1 2 5 10 Obs/Exp 0.6 0.7 0.8 0.9 1.0 GC% 30 40 50 60 70 80

General Parameters for Primer Selection Sequence name (optional): Target (optional): "start, size", such as (560, 30) Excluded Regions (optional): "start, size", such as (160, 50 1100, 50) Number of output pairs (optional): 1 2 3 4 5 6 7 8 9 Product Size: Min: Opt: Max: Primer Tm: Min: Opt: Max: Primer Size: Min: Opt: Max: Product CpGs: Primer Poly X: Primer non-CpG 'C's: Primer Poly T: Parameters for MSP primers 3'CpG constraint: Number of CpGs in primer: Max Tm difference:

Please send bug reports, feature requests to the author Long-Cheng Li.