Welcome to MethPrimer!
MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primers. Currently, it can design primers for two types of bisulfite PCR: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR. MethPrimer can also predict CpG islands in DNA sequences
Input Sequence: A DNA sequences in any format. No editing is required before input.>
Output: MethPrimer returns results in both text and graphic view including results of primer picking and CpG island prediction.
How to cite MethPrimer: Li LC and Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002 Nov;18(11):1427-31. PMID: 12424112.
MethPrimer has been cited over 1200 times according to Google Scholar. Click here to see who has used the MethPrimer program.
Submit your paper to Journal of Biological Methods (ISSN 2326-9901) - an open access journal
Methylation Method Forum
Discussion forum for methylation related methods discussion (moderated) - Join the CpG method forum to ask questions and share your knowledge on methylation related methods, such as bisulfite modification, primer design, MSP, bisulfite sequencing PCR.