MethPrimer - Design Primers for Methylation PCRs

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Welcome to MethPrimer
  • MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primers. Currently, it can design primers for two types of bisulfite PCR: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR.
  • Input Sequence: A DNA sequences in any format. No editing is required before input.
  • Output: MethPrimer returns results in both text and graphic view.

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How to cite MethPrimer: Li LC and Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002 Nov;18(11):1427-31. Click here to see who has used the MethPrimer program.

Discussion forum for methylation related methods discussion (moderated) - Join the CpG method forum to ask questions and share your knowledge on methylation related  methods, such as bisulfite modification, primer design, MSP, bisulfite sequencing PCR. 

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MethPrimer was developed by Dr. Long-Cheng Li at the Department of Urology, University of California, San Francisco.
   
To send bug reports and feature requests or contact the author, please use Feedback form.
Page last updated: July 26, 2004
visitors since Oct 16, 2002